Abstrakt

A novel antioxidant and antimicrobial compound produced by Bacillus firmicutes

Youssri M Ahmed, Ibtihaj J Jambi

In this study, antioxidant and antimicrobial compounds were produced from a dates seeds powder containing medium by B. firmicutes, a bacterial strain isolated from Al-Ain Alhara hot springs located in Gazan area in Saudi Arabia. The Total Antioxidant Capacity (TAC) of culture supernatant of B. firmicutes by the phosphomolybdenum was 2.5 mg/ml of culture medium, the total phenolic compounds is 5.3 mg/ml of culture medium and radical scavenging activity was done by DPPH. The greatest scavenging rate of antioxidants was 60%. In addition, its antibacterial activity was performed after treatment the culture medium with methanol or ethyl acetate. As antimicrobial ethyl acetate, extract is more effective than methanol. In both ethyl acetate and methanol extract the highest antibacterial activity was observed against E. faecalis with a maximum diameter inhibition zone of 25 mm, 15 mm respectively while, the lowest activity was detected against P. aeruginosa with inhibition diameter zone 14 mm and 10 mm respectively. Analysis by Thin Layer Chromatography (TLC) for ethyl acetate and methanol extracts revealed the presence one spot in the front in ethyl acetate while the spot of methanol come latter and maybe have another spot. The molecular mass of ethyl acetate extract was 304.53 m/z and methanol extract was 701.57 m/z as determined by MS.