Abstrakt

Appliance of acute phase proteins, cytokines, biogram and hemogram as markers for nanoparticles-induced clinicopathological alterations

Ibtsam Gheith

Objective: The goal of this study is to assess and confirm the clinical importance of measurement of positive and negative acute phase proteins parameters as biomarkers for inflammation; and alterations in blood picture induced by nanoparticles instead of back to tissue biopsy and histopathology.

Methods: Rats were randomly grouped into three groups, 1st group acted as control group and received 1 ml/d distilled water; the 2nd group received 1 ml of distilled water containing iron oxide at 100 μg/kg concentrations using stomach tube; while the 3rd group received 1 ml of distilled water containing iron oxide at 150 μg/kg concentrations, for a period of 4 weeks. The clinic-pathological effects were observed by estimating the alterations in different functional parameters of the liver in serum using chemical kits and in the hematological parameters by using autohematology analyzer.

Results: Alterations were in the form of significant elevations of AST, ALT and ALP enzyme activities; significant decreases in total and conjugated bilirubin; significant increases in all positive acute phase proteins and decreases in all negative acute phase ones; with significant increases in IL-1α, IL-6 and TNF-α cytokines and a disturbance in lipogram appearing as remarkable high total lipids, cholesterol, LDL-C, VLDL-C, with significant decrease in HDL-C. These results were associated with alterations in hematological parameters and occurrence of anemia in both 2nd and 3rd groups.

Conclusion: Nanoparticles have double-edged sword that ultra-structure of these articles seem to have advantage in the treatment, but in the same time may cause marked injuries to the vital organs as liver and related functions.