Abstrakt

Association of PTEN and Cyclin D1 expression with GH3 tumor cell proliferation and apoptosis in pituitary

Juexian Xiao, Jun Liu, Yaxu Shen, Shigang Lv, Jianghao Li, Zujue Cheng

Objective: To investigate the relationship between the expression of PTEN and Cyclin D1 in pituitary tumors and the proliferation and apoptosis of pituitary tumor cells.

Methods: The pGPU6-GFP-Neo-PTEN/Cyclin D1 vector was constructed with pGPU6-GFP-Neo as the vector, and the low expression of PTEN and high expression of Cyclin D1 were established. MTT assay and soft agar colony formation assay were used to detect the growth curve change and clonogenic ability. Western blot was used to detect inhibition of PTEN and Cyclin D1 and its relationship with Cyclin D1, CDK4, CDK2 and Cyclin E expression.

Results: Compared with the low PTEN expression group, the cell growth ability of the high PTEN expression group was significantly lower (p<0.05). The clonogenic ability significantly decreased with high expression of PTEN (p<0.01). The expression of Cyclin D1, CDK4, CDK2 and Cyclin E also significantly increased (p<0.01).

Conclusion: PTEN and Cyclin D1 play important roles in the proliferation of pituitary tumor cells through up-regulation of cell cycle-associated protein Cyclin D1, CDK4, CDK2 and Cyclin E.