Abstrakt

Effect of sodium ferulate on IL-8 expression induced by Ox-LDL in human umbilical vein endothelial cells

Junliang Tao, Dongxian Zhang, Dongyi Zhang, Yonghong Man, Yongyi Bi

In the present study we try to investigate the effect of Sodium Ferulate (SF) on Oxidized Low-Density Lipoprotein (Ox-LDL) induced IL-8 expression in Human Umbilical Vein Endothelial Cells (HUVEC). HUVECs were cultured in vitro and gene chip assay was used to analyse the regulation of IL-8 gene expression in HUVEC after the stimulation with Ox-LDL and SF+Ox-LDL, respectively. The IL-8 mRNA expression was measured by real-time PCR assay and protein expression by Western and ELISA assay. The results indicated that the expression of IL-8 gene was up-regulated by 6.1 fold after stimulation by Ox-LDL in HUVECs compared with that of the control. However, the expression of IL-8 gene was down-regulated by 3.3 fold after the stimulation by SF+Ox-LDL in HUVECs compared with that of in Ox-LDL treatment group. The real-time PCR results, Western and ELISA results showed that both the mRNA level and protein level of IL-8 increased significantly in Ox-LDL group compared with that of the control, and the expression decreased obviously in SF+Ox-LDL group compared with that of the Ox-LDL group. Take it together; Ox-LDL could induce the expression of IL-8, which could be inhibited by SF. Moreover, SF down-regulated the expression of IL-8, which could reduce the inflammatory response in HUVECs.