Abstrakt

ePARTS: An easy way to store and access your MoClo construct information in plant biotechnology projects.

Caro E and Pardo J

The use of modular cloning techniques like MoClo has become very popular in plant biotechnology projects that deal with the creation of multigenic constructs. The storage of the complex information related to these constructs is absolutely essential and we found that there are no free tools designed specifically for MoClo constructs available. We have designed a relational database that represents the data of interest and how they are related. A program developed in Python programming language - ePARTS - was written to make the database accessible to a broad range of users. It includes a visual interface that allows to insert and search constructs and other items as primers, enzymes or antibiotic resistances in a user friendly way. The source code of the developed system and the database is freely available to allow plant biotech community that deals with complex MoClo constructs benefit from it.