Abstrakt

Expression of lncRNA Novlnc6 in plasma and its clinical correlation with coronary artery disease

Jialong Zhu, Liang Hou, Zhijun Zhu, Siyuan Hu, Laibo Yin

Objective: To investigate the expression of lncRNA Novlnc6 in patients with different diseases and to explore the correlation between expression level of lncRNA Novlnc6 and Coronary Artery Disease (CAD).

Methods: Plasma samples were prepared using the blood extracted from 28 CAD patients and plasma samples of 28 healthy people as well as patients with a variety of different diseases. In addition, the heart tissues were collected from both the lesion area and surrounding healthy area of 7 patients with CAD. The expression levels of lncRNA Novlnc6 in plasma and heart tissue were detected using quantitative real time polymerase chain reaction (qRT-PCR).

Results: The expression of lncRNA Novlnc6 were significantly decrease in the plasma of patients with CAD and dilated cardiomyopathy compared with the healthy control, while the decrease in CAD patients were significantly higher than that in dilated cardiomyopathy patients. lncRNA Novlnc6 in plasma showed high storage stability and freeze-thaw stability. The expression of lncRNA Novlnc6 was down regulated in both plasma and heart lesion tissues. However, the decrease in plasma was significantly higher than that in heart lesion tissues.

Conclusion: The down regulation of lncRNA Novlnc6 in plasma can be used as a biomarker for the diagnosis of CAD.