Abstrakt

Expression of thrombomodulin in lung tissue cells of ALI/ARDS rats induced by severe sepsis

Lei Lirong, Wu Yan, Zhan Jia, He Xianghu, Wang Yanlin

Objective: To investigate the expression of Thrombomodulin (TM) in lung tissue cells of model rats by using the ALI/ARDS rats induced by severe sepsis as a research model.

Methods: The ALI/ARDS (Acute Lung Injury/Acute Respiratory Distress Syndrome) model rats were prepared by the cecal ligation and puncture method in combination with 0.9% sodium chloride saline solution lavage, and 22 rats were randomly divided into the sham operation group and ALI/ARDS group. After successful modeling, rats were sacrificed in 1 h, 2 h and 3 h, and quantitative lung tissues were used to prepare the tissue homogenate, and then the expression of TM was measured by Enzyme- Linked Immunosorbent Assay (ELISA).

Results: The expressions of TM in the sham operation groups were (541 ± 15 ng/ml), (733 ± 27 ng/ml), (671 ± 34 ng/ml) at 1, 2, and 3 h respectively, in the ALI/ARDS group, the expressions of TM in 1 h, 2 h and 3 h after lavage were (16790 ± 155 ng/ml), (10034 ± 281 ng/ml) and (11698 ± 195 ng/ml), respectively. By comparison, the expression in the ALI/ARDS group was significantly higher than that of the control group, showing significant difference between groups (P<0.05). There was no significant difference in all time points in the groups (P>0.05), but the animals in the two groups showed a significant increase in stress at 1 h, and continued decrease at 2 h, and then increase at 3 h.

Conclusions: The expression of TM in the lung tissue cells of ALI/ARDS rats induced by severe sepsis was high, indicating that it has obvious effect of anticoagulation and wide application values in the clinical treatment, thus, it is worthy of promotion.