Abstrakt

Inhibitory effect of emodin on human colon cancer SW620 cells and possible mechanisms

Jianfeng Zhao, Jian Xu, Jian Zhao, Rui Zhang

This study aimed to investigate the inhibitory effect of emodin on human colon cancer SW620 cells and the possible mechanisms. SW620 cells were cultured and treated with emodin with concentration of 0, 10, 20, 40, 80, 160 μmol/L. After treatment, the cell proliferation and apoptosis were detected. The expressions of B-Cell Lymphoma-2 (Bcl-2), Bcl-2 associated X (Bax) and p53 protein in cells were determined. Results showed that, emodin with all concentration could inhibit the proliferation of SW620 cells. The apoptosis rates of SW620 cells in 20, 40, 80 and 160 μmol/L emodin group were significantly higher than 0 and 10 μmol/L emodin group, respectively (P<0.05). The percentage of SW620 cells in G0/G1 phase in 10, 20, 40, 80 and 160 μmol/L emodin group were significantly higher than 0 μmol/L emodin group, respectively (P<0.05), and the percentage of SW620 cells in S phase in 10, 20, 40, 80 and 160 μmol/L emodin group were significantly lower than 0 μmol/L emodin group, respectively (P<0.05). Bcl-2 protein expression level of in 20, 40, 80 and 160 μmol/L emodin group was significantly lower than 0 and 10 μmol/L emodin group, respectively (P<0.05). Bax protein expression level in 40, 80 and 160 μmol/L emodin group was significantly higher than that in 0, 10 and 20 μmol/L emodin group, respectively (P<0.05). p53 protein expression level in 10, 20, 40 and 80 and 160 μmol/L emodin was significantly higher than 0 μmol/L emodin group, respectively (P<0.05). In conclusion, emodin has inhibitory effect on the growth of SW620 cells. This effect may be related to its regulation on Bcl-2, Bax and p53 expressions in cells.