Abstrakt

Knockdown of ET-1 gene can inhibit the proliferation, invasion of human prostate cancer cell

Jie Liu, Xiuheng Liu

Prostate cancer is the second most frequently diagnosed cancer and the sixth most commonly fatal cancer in men worldwide. Endothelin-1 (ET-1), produced by the prostate epithelia, likely plays an important role in the progression of prostate cancer. ET-1 has been previously reported in prostate and other organs. However, ET-1 expression in Prostate cancer cell lines has not been described. Therefore, we have used Western Blot to demonstrate ET-1 expression in breast cancer cell lines (LNCap cell line, PC3 cell line and DU145 cell line). The results suggest the expression of ET-1 is significantly high expressed in PC3 cell line. Moreover, knockdown of ET-1 gene with siRNA can effectively repress the cell proliferation and the cell cycle was blocked in G1/G2 phase. Knockdown of ET-1 gene can affect cell invasion, which is confirmed by cell invasion and migration related genes using west blot (-catenin, Ecadherin, RhoC and RhoA). Characterization of the role of ET-1 in the development of tumors could lead to a better understanding of the changes occurring at the molecular level during the development and progression of primary human Prostate cancer.