Abstrakt

Ovarian hyperstimulation syndrome model in mice: effect of GnRH agonist

Younes Moradi, Sara Hosseini, Samira Mohammadi-Yeganeh, Saghar Salehpour, Mohammad Salehi

Introduction: Ovarian Hyperstimulation Syndrome (OHSS) is one of the most severe complications caused by ovarian stimulation during which the ovaries become severely stimulated and endanger an individual's health. Since the negative effects of OHSS are not really clear, an animal model was needed in order to evaluate OHSS effects on the embryo. Previous studies failed to produce any OHSS model in mouse with a large amount of oocyte. The present study aimed to evaluate the role of GnRH agonist on the number and maturation of oocytes and the preimplantation development of embryo in a mouse model of OHSS.

Method: Mice were divided into 4 groups-superovulation, OHSS with GnRH agonist and OHSS without GnRH agonist, and control. The OHSS with GnRH agonist and OHSS without GnRH agonist groups were created using the defined protocol, and, in the following stages, the oocytes were collected after euthanizing the mice and prepared for in vitro fertilization (IVF), and after performing IVF, the preimplantation development of embryos was evaluated.

Results: The results showed that, in the OHSS mouse model, the rate of oocyte maturation significantly improved in the treated GnRH agonist group comparing to the untreated GnRH agonist group. Fertilization and preimplantation development were significantly lower in the untreated GnRH agonist OHSS group.

Conclusion: With regard to the positive effects of GnRH agonist on oocyte number, oocyte maturation and preimplantation stages of embryos of mouse model, it seems that GnRH agonist can be used in order to improve the results of fertilization in the mouse model of OHSS.