Abstrakt

Phytochemical analysis and antioxidant activity of Persia americana and Actinidia deliciosa fruit extracts by DPPH method

Sadia Rafique, Naveed Akhtar

Extracts having antioxidant properties show promising effect on human skin. The main objective of this study was to perform phytochemical analysis to identify active constituents and determine the antioxidant activity through DPPH (2, 2 diphenyl-1-picryl hydrazyl) method in sampled fruit. In this work, extract of Persia americana variety ‘Hass’ and Actinidia deliciosa fruit were selected. Various qualitative and quantitative tests were performed for phytochemical analysis. For antioxidant activity, different parts of fruit samples of Persia americana were prepared in methanol and in acetone solution while in case of Actinidia deliciosa whole fruit was soaked in methanol and in ethanol solution. Phytochemical analysis showed the higher concentration of flavonoids and phenolics contents in both fruit extracts. The analysis also showed the presence of vitamin C, alkaloids, glycosides, amino acids, carbohydrates, proteins, steroids and triterpenoids. The DPPH results using ascorbic acid as standard showed that acetone and methanol extract of Persia americana possess higher antioxidant activity (84%) than methanol extract alone (60%) while Actinidia deliciosa whole fruit showed better antioxidant activity in ethanol extract (79%) as compared to methanol extract (57%). The extracts possess antioxidant activity can be incorporated in different skin formulations and their In-vitro and In- vivo studies may be performed for cosmetic market.