Abstrakt

Polyphyllin I inhibit cell viability, migration and invasion of human colon cancer cells by regulating MiR-26a/TGF-β signaling pathway

Aixiang Wang, Can Liu, Yuan Li

miR-26a is reported to be involved in both tumor suppressor or oncogenic action in various types of cancer progression. Colon cancer is the fourth leading lethal form of cancer and cause large number of deaths worldwide. Despite its various health beneficial effects against abnormalities, Polyphyllin I (PPI), has lack of scientific evidence on its role in colon cancer therapeutic efficacy were entice our attention. This study is designed to identify the role of miR-26a in the PPI mediated treatment for colon cancer. Further, Gene Ontology (GO) data on miR-26a biological process of its association with Transforming Growth Factor (TGF-β) signaling also hypothesized. In the present study, the protective role of PPI in cell migration and invasion of human colon cancer SW-948 and HT-29 cells was determined assays method. Further, reverse transcription-quantitative Polymerase Chain Reaction (qRT-PCR) analysis was carried out to determine the expression pattern of miR-26a and TGF-β, subsequently western blot analysis was performed to determine the TGF-β expression pattern. This experimental analysis validates the therapeutic efficiency of PPI that whether the anticancer potential was mediated by Transforming Growth Factor-β (TGF-β) cascade signaling and microRNA (miR) 26a. Earlier, Cell viability assay was performed to identify the Minimum inhibitory concentration M (IC50) of PPI on colon cancer cells. Our results suggest that cell proliferation, migration and invasion of cancer cells (SW-948 and HT-29) was significantly suppressed by PPI treatment, further the effect was observed in a dose-dependent manner. Moreover, following PPI treatment, miR-26a was up-regulated were further induce the tumor suppressor property also. This process consequently alters the TGF-β signaling cascade process that reportedly complicated, were associate with cell proliferation and differentiation. Findings of this study elucidated that, the over expression of miR-26a subsequently downstream the TGF-β signaling pathway, consequence of this effect was found to be PPI treated colon cancer cells. This might be the possible mechanism of action of PPI by which appears to be produce effective therapeutic potential in colon cancer therapy.