Zeitschrift für klinische Pathologie und Labormedizin


Production of protease enzyme from fish guts using Pseudomonas fluorescens, Enterobacter cloacae and Bacillus megaterium.

Fajingbesi AO, Abbas Abel Anzaku, Akande M, Ikwue Adakole Emmanuel, Ombugadu Akwashiki

Food industry wastes are an essential environmental contamination source. A large number of microorganisms including bacteria, yeast and fungi produce different types of enzymes like protease, lipase, chitinase, chitosan and ligninolytic enzyme having high biotechnological interest such as food processing, detergent, textile, pharmaceutical products, and medical therapy. Microbial synthesis of enzymes has been reported to be influenced by various factors such as carbon sources, nitrogen sources, and operating parameters like the temperature and pH. This research has been done in order to develop methods to convert these wastes into useful products. Three proteolytic organisms (Pseudomonas fluorescens, Enterobacter cloacae and Bacillus megaterium) were used in the production of protease and the most effective production was observed at a temperature of 45°C and pH 9. P. fluorescens produced protease best at 3rd production of 48 and 72 hours, E. cloacae produced protease best at 3rd production at 72 hours and B. megaterium produced best at 24 hours of 3rd production. P. fluorescens, E. cloacae and B. megaterium can produce protease and can therefore be used in the degradation of fish waste. These enzymes can also be used medically to control blood clotting target for degrading pathogenic proteins.