Abstrakt

Reduced expression level of miR-205 predicts poor prognosis in osteosarcoma

Qiang Yang, Peng Sun, Haotian Feng, Xin Li, Jianmin Li

Objective: MicroRNA-205 (miR-205), acts as an oncogene or tumor suppressor by modulating the expression of multiple cancer-related target genes. In the present study, we aimed to investigate the clinical significance and prognostic value of miR-205 in osteosarcoma patients.

Methods: Expression level of miR-205 was evaluated by qRT-PCR in osteosarcoma tissues and adjacent non-neoplastic tissues. The relationship between the clinicopathological characteristics and miR-205 was estimated by chi-square test. Associations between overall survival and miR-205 expression were evaluated using Kaplan-Meier analysis according to log rank test. Cox regression was carried out to determine the prognostic effects of clinicopathological characteristics.

Results: The expression levels of miR-205 in osteosarcoma tissues were significantly lower than those in noncancerous bone tissues (P<0.001). Low miR-205 expression was significantly associated with patients with advanced clinical stage (P=0.001) and presence of distant metastasis (P<0.001). We found that the survival time of patients with low miR-205 expression was shorter than those with high miR-205 expression (P=0.046). Furthermore, the multivariate analysis with Cox’s proportional hazards model confirmed that low miR-205 expression level was an independent predictor of poor prognosis for the osteosarcoma patients (Hazard ration=2.885; 95% CI: 1.479-8.492, P=0.037).

Conclusions: The present study showed that miR-205 might be a promising biomarker for the detection of osteosarcoma and its down-regulation might be potentially associated with unfavorable prognosis of osteosarcoma.