Abstrakt

Role of Wnt/β-catenin signaling pathway in migration of asthmatic rat airway smooth muscle cells

Xiaomei Fang, Jianjiang Zhao, Lina Shen, Miaofen Zheng, Hong Zhou, Guofang Tao, Zheng Wu, Yuhua Ge

Objective: To investigate the role of Wnt/β-catenin signaling pathway on migration ability in Asthmatic Rat Airway Smooth Muscle Cells (ASMCs).

Methods: The asthmatic rat airway smooth muscle cells were cultured in vitro. The expression of Wnt/β- catenin in ASMCs was detected by RT-PCR and Western blot. Migration ability of ASMCs was detected by wound healing assay and Transwell migration assay.

Results: Compared with normal rats, the expression level of Wnt/β-catenin in chronic asthmatic rats ASMCs was obvious higher. Knockdown of Wnt/β-catenin signaling pathway by specific siRNA was able to decreases the migration ability of ASMCs.

Conclusion: The migration ability of ASMCs of chronic asthma rats’ increases, and Wnt/β-catenin signaling pathway may participants in this event.