Abstrakt
Study of biomarkers involved in amyloidgenic pathway for over-production of amyloid beta leading to alzheimerâs disease.
Divya Jindal*
APP is an Amyloid Precursor Protein which is present endogenously in many parts of the body, is cleaved by enzyme β-secretase (a transmembrane aspartic protease enzyme- BACE). When APP is cleaved by this enzyme, the protein is divided into two parts i.e. Soluble sAPPβ and membrane bound CTFβ. There is an enzyme γ-secretase which cleaves this CTFβ structure, dividing into Amyloid Beta peptides and membrane bound fragment AICD. Since, Amyloid beta (Aβ) is required for neural functioning but Cluster formation of these Amyloid beta peptides leads to form plaques that interrupts the Brain function, finally causing neural cell death. These plaques disturb the brain cells by clogging the cell junctions and hence activating immune cells for inflammation which become lethal to cells. Hence, two key biomarkers are studied with the help of Western Blotting to check their activity and over production in normal cells so as to study the change after being diseased.