Abstrakt

Vitamin E TPGS based liposomal delivery of doxorubicin in osteosarcoma cancer cells

Yu-Xin Zhao, Cai- Li Guo, Wei-Tao Yao, Qi-Qin Cai, Yi-Sheng Wang, Jia- Qiang Wang

This research work has been carried out in order to explore the role of Vitamin E TPGS in osteosarcoma (OS). We have prepared TPGS grafted liposome’s loaded with Doxorubicin (TPGS-Lip-DOX) and explored its effect on OS cells. The prepared TPGS liposomes have been characterized for its size, charge, percentage entrapment efficiency. The TPGS-Lip-DOX showed a high entrapment efficiency of 71.5 ± 3.9% with 225 ± 15.6 nm size with homogeneous particles dispersion of PDI, 0.034 ± 0.008. TPGSLip- DOX has showed a sustained release pattern. The cytotoxicity tests exposed that the non-toxicity of control liposome’s and TPGS-Lip-DOX showed concentration dependent toxicity. It was evident that TPGS exerted increased oxidative effect on MG63 cells and TPGS-Lip-DOX facilitates highly lethal effect on MG63 cells and causes apoptosis. The results conclude that the role of TPGS is synergistic as an effective delivery system for cancer chemotherapy