Abstrakt

Biochemistry 2021: CRP promoter response to complete freund's adjuvant immune stimulation- Lubomir Ondruska, V Parkanyi, D Vasicek, R Jurcik, E Hanusova, J Vasicek and A Balazi- National Agricultural and Food Centre, Slovak Republic

Lubomir Ondruska, V Parkanyi, D Vasicek, R Jurcik, E Hanusova, J Vasicek and A Balaz

The injections of adjuvants to animals often induce inflammatory reactions. Inflammatory reactions are one of the potential safety concerns that are evaluated in the framework of vaccine safety testing. In nonclinical studies, the assessment of the inflammation relies notably on the measurement of biomarkers like C-reactive protein (CRP). This study was aimed at evaluating the respective value of inflammation biomarker in different rabbit CRP promoter genotypes. Together 60 of crossbred rabbit line, based on New Zealand white rabbits different CRP promoter genotypes -119AC and -119AA (identified SNPs from start of transcription) were analyzed. In the 60 days of age all rabbits were subcutaneously injected with 0.125 ml (per kg of live weight) of Complete Freund's Adjuvant (CFA), with content of 125