Abstrakt

Generation of a recombinant antimicrobial peptide in transgenic plants employing an adjusted VMA intein expression framework

Stefano Marchett

Tobacco plants were designed to specific SMAP-29, a mammalian antimicrobial peptide of natural resistance, as combination protein with adjusted vacuolar film ATPase intein. The peptide was filtered taking advantage of the intein-mediated self-cleaving mechanism. SMAP29 was immunologically identified within the chromatographic eluate and showed up firmly bound to copurified plant proteins. Electrophoretic division beneath disaggregating conditions demonstrated that the recombinant peptide was cleaved off by intein at the anticipated location and an overlay gel measure illustrated that the peptide held antimicrobial action. These comes about demonstrate that an adjusted intein expression framework can be utilized to create pharmaceutical peptides in transgenic plants.